Construct: shRNA TRCN0000118612

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153240.3-4177s1c1
DNA Barcode:
CGTGTGATTTAACTGCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPHP3 (27031)
Gene Description:
nephrocystin 3
Transcript:
RefSeq NM_153240.3 (NON-CURRENT)
Match location:
Position 4177 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118612)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTGATTTAACTGCTATTT
Hairpin Sequence:
5'-CCGG-CGTGTGATTTAACTGCTATTT-CTCGAG-AAATAGCAGTTAAATCACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTGATTTAACTGCTATTTCTCGAGAAATAGCAGTTAAATCACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTGATTTAACTGCTATTTCTCGAGAAATAGCAGTTAAATCACACG-3'

Other clones with same target sequence:

(none)