Construct: shRNA TRCN0000118647

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005647.2-3289s1c1
DNA Barcode:
GCGTAGTAAGTGCTTTATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBL1X (6907)
Gene Description:
transducin beta like 1 X-linked
Transcript:
RefSeq NM_005647.2 (NON-CURRENT)
Match location:
Position 3289 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118647)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTAGTAAGTGCTTTATGTA
Hairpin Sequence:
5'-CCGG-GCGTAGTAAGTGCTTTATGTA-CTCGAG-TACATAAAGCACTTACTACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTAGTAAGTGCTTTATGTACTCGAGTACATAAAGCACTTACTACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTAGTAAGTGCTTTATGTACTCGAGTACATAAAGCACTTACTACGC-3'

Other clones with same target sequence:

  1. TRCN0000310425