Construct: shRNA TRCN0000118689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001976.2-939s1c1
DNA Barcode:
AGAGCTGTATAAGAGCTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ENO3 (2027)
Gene Description:
enolase 3
Transcript:
RefSeq NM_001976.2 (NON-CURRENT)
Match location:
Position 939 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCTGTATAAGAGCTTTAT
Hairpin Sequence:
5'-CCGG-AGAGCTGTATAAGAGCTTTAT-CTCGAG-ATAAAGCTCTTATACAGCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCTGTATAAGAGCTTTATCTCGAGATAAAGCTCTTATACAGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCTGTATAAGAGCTTTATCTCGAGATAAAGCTCTTATACAGCTCT-3'

Other clones with same target sequence:

(none)