Construct: shRNA TRCN0000118719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003023.2-1773s1c1
DNA Barcode:
CTCTAACAAAGTGAGGAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BP2 (6452)
Gene Description:
SH3 domain binding protein 2
Transcript:
RefSeq NM_003023.2 (NON-CURRENT)
Match location:
Position 1773 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTAACAAAGTGAGGAACTA
Hairpin Sequence:
5'-CCGG-CTCTAACAAAGTGAGGAACTA-CTCGAG-TAGTTCCTCACTTTGTTAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTAACAAAGTGAGGAACTACTCGAGTAGTTCCTCACTTTGTTAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTAACAAAGTGAGGAACTACTCGAGTAGTTCCTCACTTTGTTAGAG-3'

Other clones with same target sequence:

(none)