Construct: shRNA TRCN0000118752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015915.2-2274s1c1
DNA Barcode:
GCTGGATTTAATCTGTATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATL1 (51062)
Gene Description:
atlastin GTPase 1
Transcript:
RefSeq NM_015915.2 (NON-CURRENT)
Match location:
Position 2274 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATTTAATCTGTATCAT
Hairpin Sequence:
5'-CCGG-GCTGGATTTAATCTGTATCAT-CTCGAG-ATGATACAGATTAAATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATTTAATCTGTATCATCTCGAGATGATACAGATTAAATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATTTAATCTGTATCATCTCGAGATGATACAGATTAAATCCAGC-3'

Other clones with same target sequence:

(none)