Construct: shRNA TRCN0000118765

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000287.2-683s1c1
DNA Barcode:
CCAGAGAGTCATCGAACACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PEX6 (5190)
Gene Description:
peroxisomal biogenesis factor 6
Transcript:
RefSeq NM_000287.2 (NON-CURRENT)
Match location:
Position 683 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118765)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGAGTCATCGAACACTT
Hairpin Sequence:
5'-CCGG-CCAGAGAGTCATCGAACACTT-CTCGAG-AAGTGTTCGATGACTCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGAGTCATCGAACACTTCTCGAGAAGTGTTCGATGACTCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGAGTCATCGAACACTTCTCGAGAAGTGTTCGATGACTCTCTGG-3'

Other clones with same target sequence:

(none)