Construct: shRNA TRCN0000118912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002973.1-2293s1c1
DNA Barcode:
GCTGGTATTATTCCAACTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATXN2 (6311)
Gene Description:
ataxin 2
Transcript:
RefSeq NM_002973.1 (NON-CURRENT)
Match location:
Position 2293 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTATTATTCCAACTGAA
Hairpin Sequence:
5'-CCGG-GCTGGTATTATTCCAACTGAA-CTCGAG-TTCAGTTGGAATAATACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTATTATTCCAACTGAACTCGAGTTCAGTTGGAATAATACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTATTATTCCAACTGAACTCGAGTTCAGTTGGAATAATACCAGC-3'

Other clones with same target sequence:

(none)