Construct: shRNA TRCN0000118999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018662.1-2405s1c1
DNA Barcode:
CATAGGCAAGAAGCTATTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DISC1 (27185)
Gene Description:
DISC1 scaffold protein
Transcript:
RefSeq NM_018662.1 (NON-CURRENT)
Match location:
Position 2405 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGGCAAGAAGCTATTGTA
Hairpin Sequence:
5'-CCGG-CATAGGCAAGAAGCTATTGTA-CTCGAG-TACAATAGCTTCTTGCCTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGGCAAGAAGCTATTGTACTCGAGTACAATAGCTTCTTGCCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGGCAAGAAGCTATTGTACTCGAGTACAATAGCTTCTTGCCTATG-3'

Other clones with same target sequence:

(none)