Construct: shRNA TRCN0000119061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015570.1-2621s1c1
DNA Barcode:
CTTCCGTTACACCCAACTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AUTS2 (26053)
Gene Description:
activator of transcription and developmental regulator AUTS2
Transcript:
RefSeq NM_015570.1 (NON-CURRENT)
Match location:
Position 2621 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCGTTACACCCAACTCAA
Hairpin Sequence:
5'-CCGG-CTTCCGTTACACCCAACTCAA-CTCGAG-TTGAGTTGGGTGTAACGGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCGTTACACCCAACTCAACTCGAGTTGAGTTGGGTGTAACGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCGTTACACCCAACTCAACTCGAGTTGAGTTGGGTGTAACGGAAG-3'

Other clones with same target sequence:

  1. TRCN0000300577