Construct: shRNA TRCN0000119072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007166.1-1598s1c1
DNA Barcode:
GCCTTAATGTTGACTTTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PICALM (8301)
Gene Description:
phosphatidylinositol binding clathrin assembly protein
Transcript:
RefSeq NM_007166.1 (NON-CURRENT)
Match location:
Position 1598 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTAATGTTGACTTTGAAT
Hairpin Sequence:
5'-CCGG-GCCTTAATGTTGACTTTGAAT-CTCGAG-ATTCAAAGTCAACATTAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTAATGTTGACTTTGAATCTCGAGATTCAAAGTCAACATTAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTAATGTTGACTTTGAATCTCGAGATTCAAAGTCAACATTAAGGC-3'

Other clones with same target sequence:

(none)