Construct: shRNA TRCN0000119101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012120.1-199s1c1
DNA Barcode:
CTGACAATTTCGTTAAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD2AP (23607)
Gene Description:
CD2 associated protein
Transcript:
RefSeq NM_012120.1 (NON-CURRENT)
Match location:
Position 199 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACAATTTCGTTAAGGAAA
Hairpin Sequence:
5'-CCGG-CTGACAATTTCGTTAAGGAAA-CTCGAG-TTTCCTTAACGAAATTGTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACAATTTCGTTAAGGAAACTCGAGTTTCCTTAACGAAATTGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACAATTTCGTTAAGGAAACTCGAGTTTCCTTAACGAAATTGTCAG-3'

Other clones with same target sequence:

(none)