Construct: shRNA TRCN0000119116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004616.2-517s1c1
DNA Barcode:
CTGATCGCATTGTGAATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSPAN8 (7103)
Gene Description:
tetraspanin 8
Transcript:
RefSeq NM_004616.2 (NON-CURRENT)
Match location:
Position 517 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATCGCATTGTGAATGAAA
Hairpin Sequence:
5'-CCGG-CTGATCGCATTGTGAATGAAA-CTCGAG-TTTCATTCACAATGCGATCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATCGCATTGTGAATGAAACTCGAGTTTCATTCACAATGCGATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATCGCATTGTGAATGAAACTCGAGTTTCATTCACAATGCGATCAG-3'

Other clones with same target sequence:

(none)