Construct: shRNA TRCN0000119132

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371143.2-1682s1c1
DNA Barcode:
GCTTCCCAAATTGTGAGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLEC17A (388512)
Gene Description:
C-type lectin domain containing 17A
Transcript:
RefSeq XM_371143.2 (NON-CURRENT)
Match location:
Position 1682 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119132)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCCAAATTGTGAGGATT
Hairpin Sequence:
5'-CCGG-GCTTCCCAAATTGTGAGGATT-CTCGAG-AATCCTCACAATTTGGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCCAAATTGTGAGGATTCTCGAGAATCCTCACAATTTGGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCCAAATTGTGAGGATTCTCGAGAATCCTCACAATTTGGGAAGC-3'

Other clones with same target sequence:

(none)