Construct: shRNA TRCN0000119143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014917.2-1085s1c1
DNA Barcode:
CCTGGAGAAGTCTCTCGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTNG1 (22854)
Gene Description:
netrin G1
Transcript:
RefSeq NM_014917.2 (NON-CURRENT)
Match location:
Position 1085 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGAAGTCTCTCGATTA
Hairpin Sequence:
5'-CCGG-CCTGGAGAAGTCTCTCGATTA-CTCGAG-TAATCGAGAGACTTCTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGAAGTCTCTCGATTACTCGAGTAATCGAGAGACTTCTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGAAGTCTCTCGATTACTCGAGTAATCGAGAGACTTCTCCAGG-3'

Other clones with same target sequence:

(none)