Construct: shRNA TRCN0000119194

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021229.2-1946s1c1
DNA Barcode:
GCACTTCTACACTCAGGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTN4 (59277)
Gene Description:
netrin 4
Transcript:
RefSeq NM_021229.2 (NON-CURRENT)
Match location:
Position 1946 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119194)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTCTACACTCAGGTAAA
Hairpin Sequence:
5'-CCGG-GCACTTCTACACTCAGGTAAA-CTCGAG-TTTACCTGAGTGTAGAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTCTACACTCAGGTAAACTCGAGTTTACCTGAGTGTAGAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTCTACACTCAGGTAAACTCGAGTTTACCTGAGTGTAGAAGTGC-3'

Other clones with same target sequence:

(none)