Construct: shRNA TRCN0000119232

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033401.2-4252s1c1
DNA Barcode:
CATAGCATTCATTCTATGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNTNAP4 (85445)
Gene Description:
contactin associated protein like 4
Transcript:
RefSeq NM_033401.2 (NON-CURRENT)
Match location:
Position 4252 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119232)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGCATTCATTCTATGGAA
Hairpin Sequence:
5'-CCGG-CATAGCATTCATTCTATGGAA-CTCGAG-TTCCATAGAATGAATGCTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGCATTCATTCTATGGAACTCGAGTTCCATAGAATGAATGCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGCATTCATTCTATGGAACTCGAGTTCCATAGAATGAATGCTATG-3'

Other clones with same target sequence:

(none)