Construct: shRNA TRCN0000119304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019759.1-504s1c1
DNA Barcode:
GCGAGGAGCAACAACCACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpt (56429)
Gene Description:
dermatopontin
Transcript:
RefSeq NM_019759.1 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGGAGCAACAACCACTTT
Hairpin Sequence:
5'-CCGG-GCGAGGAGCAACAACCACTTT-CTCGAG-AAAGTGGTTGTTGCTCCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGGAGCAACAACCACTTTCTCGAGAAAGTGGTTGTTGCTCCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGGAGCAACAACCACTTTCTCGAGAAAGTGGTTGTTGCTCCTCGC-3'

Other clones with same target sequence:

  1. TRCN0000351956