Construct: shRNA TRCN0000119368

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153457.6-2315s1c1
DNA Barcode:
CGCATCTACAAGTCCGTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rtn1 (104001)
Gene Description:
reticulon 1
Transcript:
RefSeq NM_153457.6 (NON-CURRENT)
Match location:
Position 2315 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119368)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATCTACAAGTCCGTTCTA
Hairpin Sequence:
5'-CCGG-CGCATCTACAAGTCCGTTCTA-CTCGAG-TAGAACGGACTTGTAGATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATCTACAAGTCCGTTCTACTCGAGTAGAACGGACTTGTAGATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATCTACAAGTCCGTTCTACTCGAGTAGAACGGACTTGTAGATGCG-3'

Other clones with same target sequence:

(none)