Construct: shRNA TRCN0000119393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172406.2-315s1c1
DNA Barcode:
CCACAGTATAAGCTAAGAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trak2 (70827)
Gene Description:
trafficking protein, kinesin binding 2
Transcript:
RefSeq NM_172406.2 (NON-CURRENT)
Match location:
Position 315 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGTATAAGCTAAGAGTA
Hairpin Sequence:
5'-CCGG-CCACAGTATAAGCTAAGAGTA-CTCGAG-TACTCTTAGCTTATACTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGTATAAGCTAAGAGTACTCGAGTACTCTTAGCTTATACTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGTATAAGCTAAGAGTACTCGAGTACTCTTAGCTTATACTGTGG-3'

Other clones with same target sequence:

(none)