Construct: shRNA TRCN0000119405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003930.1-892s1c1
DNA Barcode:
CGGGATCAGACCAAGTCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rtn3 (20168)
Gene Description:
reticulon 3
Transcript:
RefSeq NM_001003930.1 (NON-CURRENT)
Match location:
Position 892 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATCAGACCAAGTCAATT
Hairpin Sequence:
5'-CCGG-CGGGATCAGACCAAGTCAATT-CTCGAG-AATTGACTTGGTCTGATCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATCAGACCAAGTCAATTCTCGAGAATTGACTTGGTCTGATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATCAGACCAAGTCAATTCTCGAGAATTGACTTGGTCTGATCCCG-3'

Other clones with same target sequence:

  1. TRCN0000349476