Construct: shRNA TRCN0000119414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025751.1-583s1c1
DNA Barcode:
GCCACAGTTAATAAGGCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4933425L06Rik (66763)
Gene Description:
RIKEN cDNA 4933425L06 gene
Transcript:
RefSeq NM_025751.1 (NON-CURRENT)
Match location:
Position 583 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACAGTTAATAAGGCCAAT
Hairpin Sequence:
5'-CCGG-GCCACAGTTAATAAGGCCAAT-CTCGAG-ATTGGCCTTATTAACTGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACAGTTAATAAGGCCAATCTCGAGATTGGCCTTATTAACTGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACAGTTAATAAGGCCAATCTCGAGATTGGCCTTATTAACTGTGGC-3'

Other clones with same target sequence:

(none)