Construct: shRNA TRCN0000119417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011986.2-2694s1c1
DNA Barcode:
CCACCTCTCAAGTATAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncdn (26562)
Gene Description:
neurochondrin
Transcript:
RefSeq NM_011986.2 (NON-CURRENT)
Match location:
Position 2694 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTCTCAAGTATAAGAAA
Hairpin Sequence:
5'-CCGG-CCACCTCTCAAGTATAAGAAA-CTCGAG-TTTCTTATACTTGAGAGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTCTCAAGTATAAGAAACTCGAGTTTCTTATACTTGAGAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTCTCAAGTATAAGAAACTCGAGTTTCTTATACTTGAGAGGTGG-3'

Other clones with same target sequence:

(none)