Construct: shRNA TRCN0000119443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010769.1-1078s1c1
DNA Barcode:
GCCGCCTTGAAGTATCTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Matn1 (17180)
Gene Description:
matrilin 1, cartilage matrix protein
Transcript:
RefSeq NM_010769.1 (NON-CURRENT)
Match location:
Position 1078 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCCTTGAAGTATCTCATA
Hairpin Sequence:
5'-CCGG-GCCGCCTTGAAGTATCTCATA-CTCGAG-TATGAGATACTTCAAGGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCCTTGAAGTATCTCATACTCGAGTATGAGATACTTCAAGGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCCTTGAAGTATCTCATACTCGAGTATGAGATACTTCAAGGCGGC-3'

Other clones with same target sequence:

(none)