Construct: shRNA TRCN0000119459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175425.3-1200s1c1
DNA Barcode:
GACAGCTTGTTCTCTGGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C1qtnf7 (109323)
Gene Description:
C1q and tumor necrosis factor related protein 7
Transcript:
RefSeq NM_175425.3 (NON-CURRENT)
Match location:
Position 1200 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGCTTGTTCTCTGGGTTT
Hairpin Sequence:
5'-CCGG-GACAGCTTGTTCTCTGGGTTT-CTCGAG-AAACCCAGAGAACAAGCTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGCTTGTTCTCTGGGTTTCTCGAGAAACCCAGAGAACAAGCTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGCTTGTTCTCTGGGTTTCTCGAGAAACCCAGAGAACAAGCTGTC-3'

Other clones with same target sequence:

(none)