Construct: shRNA TRCN0000119520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026799.2-1815s1c1
DNA Barcode:
CGAAGAGATGATGCCTGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Drosha (14000)
Gene Description:
drosha, ribonuclease type III
Transcript:
RefSeq NM_026799.2 (NON-CURRENT)
Match location:
Position 1815 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGAGATGATGCCTGAGAA
Hairpin Sequence:
5'-CCGG-CGAAGAGATGATGCCTGAGAA-CTCGAG-TTCTCAGGCATCATCTCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGAGATGATGCCTGAGAACTCGAGTTCTCAGGCATCATCTCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGAGATGATGCCTGAGAACTCGAGTTCTCAGGCATCATCTCTTCG-3'

Other clones with same target sequence:

(none)