Construct: shRNA TRCN0000119612

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178886.1-2359s1c1
DNA Barcode:
CCCTTCTAGTTAAGGGACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ldlrad3 (241576)
Gene Description:
low density lipoprotein receptor class A domain containing 3
Transcript:
RefSeq NM_178886.1 (NON-CURRENT)
Match location:
Position 2359 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119612)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCTAGTTAAGGGACTAT
Hairpin Sequence:
5'-CCGG-CCCTTCTAGTTAAGGGACTAT-CTCGAG-ATAGTCCCTTAACTAGAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCTAGTTAAGGGACTATCTCGAGATAGTCCCTTAACTAGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCTAGTTAAGGGACTATCTCGAGATAGTCCCTTAACTAGAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000153529