Construct: shRNA TRCN0000119648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019802.1-111s1c1
DNA Barcode:
GCCTCAGATAAAGTACAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ggcx (56316)
Gene Description:
gamma-glutamyl carboxylase
Transcript:
RefSeq NM_019802.1 (NON-CURRENT)
Match location:
Position 111 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAGATAAAGTACAGAAA
Hairpin Sequence:
5'-CCGG-GCCTCAGATAAAGTACAGAAA-CTCGAG-TTTCTGTACTTTATCTGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAGATAAAGTACAGAAACTCGAGTTTCTGTACTTTATCTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAGATAAAGTACAGAAACTCGAGTTTCTGTACTTTATCTGAGGC-3'

Other clones with same target sequence:

  1. TRCN0000323596