Construct: shRNA TRCN0000119734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173866.1-873s1c1
DNA Barcode:
CCCTAAAGTTCTCTGCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpt2 (108682)
Gene Description:
glutamic pyruvate transaminase (alanine aminotransferase) 2
Transcript:
RefSeq NM_173866.1 (NON-CURRENT)
Match location:
Position 873 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAAAGTTCTCTGCATTAT
Hairpin Sequence:
5'-CCGG-CCCTAAAGTTCTCTGCATTAT-CTCGAG-ATAATGCAGAGAACTTTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAAAGTTCTCTGCATTATCTCGAGATAATGCAGAGAACTTTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAAAGTTCTCTGCATTATCTCGAGATAATGCAGAGAACTTTAGGG-3'

Other clones with same target sequence:

(none)