Construct: shRNA TRCN0000119770

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027907.1-372s1c1
DNA Barcode:
CACGACAACATCATTGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Etnppl (71760)
Gene Description:
ethanolamine phosphate phospholyase
Transcript:
RefSeq NM_027907.1 (NON-CURRENT)
Match location:
Position 372 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119770)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGACAACATCATTGAGTTT
Hairpin Sequence:
5'-CCGG-CACGACAACATCATTGAGTTT-CTCGAG-AAACTCAATGATGTTGTCGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGACAACATCATTGAGTTTCTCGAGAAACTCAATGATGTTGTCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGACAACATCATTGAGTTTCTCGAGAAACTCAATGATGTTGTCGTG-3'

Other clones with same target sequence:

(none)