Construct: shRNA TRCN0000119957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011917.1-1513s1c1
DNA Barcode:
CCAGAGGATAACGTCAGGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xrn2 (24128)
Gene Description:
5'-3' exoribonuclease 2
Transcript:
RefSeq NM_011917.1 (NON-CURRENT)
Match location:
Position 1513 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGGATAACGTCAGGTTA
Hairpin Sequence:
5'-CCGG-CCAGAGGATAACGTCAGGTTA-CTCGAG-TAACCTGACGTTATCCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGGATAACGTCAGGTTACTCGAGTAACCTGACGTTATCCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGGATAACGTCAGGTTACTCGAGTAACCTGACGTTATCCTCTGG-3'

Other clones with same target sequence:

(none)