Construct: shRNA TRCN0000119999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178617.3-405s1c1
DNA Barcode:
CCACACCATTGACACACACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Necab1 (69352)
Gene Description:
N-terminal EF-hand calcium binding protein 1
Transcript:
RefSeq NM_178617.3 (NON-CURRENT)
Match location:
Position 405 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000119999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACACCATTGACACACACAA
Hairpin Sequence:
5'-CCGG-CCACACCATTGACACACACAA-CTCGAG-TTGTGTGTGTCAATGGTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACACCATTGACACACACAACTCGAGTTGTGTGTGTCAATGGTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACACCATTGACACACACAACTCGAGTTGTGTGTGTCAATGGTGTGG-3'

Other clones with same target sequence:

(none)