Construct: shRNA TRCN0000120003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021546.1-220s1c1
DNA Barcode:
GACCAGTTTGTCACACGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Necab3 (56846)
Gene Description:
N-terminal EF-hand calcium binding protein 3
Transcript:
RefSeq NM_021546.1 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCAGTTTGTCACACGATTT
Hairpin Sequence:
5'-CCGG-GACCAGTTTGTCACACGATTT-CTCGAG-AAATCGTGTGACAAACTGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCAGTTTGTCACACGATTTCTCGAGAAATCGTGTGACAAACTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCAGTTTGTCACACGATTTCTCGAGAAATCGTGTGACAAACTGGTC-3'

Other clones with same target sequence:

(none)