Construct: shRNA TRCN0000120023

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028291.3-305s1c1
DNA Barcode:
GCCACGATCTTCTGACTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pan3 (72587)
Gene Description:
PAN3 poly(A) specific ribonuclease subunit
Transcript:
RefSeq NM_028291.3 (NON-CURRENT)
Match location:
Position 305 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120023)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACGATCTTCTGACTTCAT
Hairpin Sequence:
5'-CCGG-GCCACGATCTTCTGACTTCAT-CTCGAG-ATGAAGTCAGAAGATCGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACGATCTTCTGACTTCATCTCGAGATGAAGTCAGAAGATCGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACGATCTTCTGACTTCATCTCGAGATGAAGTCAGAAGATCGTGGC-3'

Other clones with same target sequence:

(none)