Construct: shRNA TRCN0000120046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024433.1-519s1c1
DNA Barcode:
CCTCATAGAAACTGCTAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtap (66902)
Gene Description:
methylthioadenosine phosphorylase
Transcript:
RefSeq NM_024433.1 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATAGAAACTGCTAAGAA
Hairpin Sequence:
5'-CCGG-CCTCATAGAAACTGCTAAGAA-CTCGAG-TTCTTAGCAGTTTCTATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATAGAAACTGCTAAGAACTCGAGTTCTTAGCAGTTTCTATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATAGAAACTGCTAAGAACTCGAGTTCTTAGCAGTTTCTATGAGG-3'

Other clones with same target sequence:

  1. TRCN0000336134