Construct: shRNA TRCN0000120063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019975.2-933s1c1
DNA Barcode:
GCGAGACTAAACTGGATCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hacl1 (56794)
Gene Description:
2-hydroxyacyl-CoA lyase 1
Transcript:
RefSeq NM_019975.2 (NON-CURRENT)
Match location:
Position 933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGACTAAACTGGATCTTA
Hairpin Sequence:
5'-CCGG-GCGAGACTAAACTGGATCTTA-CTCGAG-TAAGATCCAGTTTAGTCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGACTAAACTGGATCTTACTCGAGTAAGATCCAGTTTAGTCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGACTAAACTGGATCTTACTCGAGTAAGATCCAGTTTAGTCTCGC-3'

Other clones with same target sequence:

(none)