Construct: shRNA TRCN0000120089

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145953.2-1131s1c1
DNA Barcode:
CGGGATCAATGACACACTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cth (107869)
Gene Description:
cystathionase (cystathionine gamma-lyase)
Transcript:
RefSeq NM_145953.2 (CURRENT)
Match location:
Position 1131 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120089)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATCAATGACACACTGAT
Hairpin Sequence:
5'-CCGG-CGGGATCAATGACACACTGAT-CTCGAG-ATCAGTGTGTCATTGATCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATCAATGACACACTGATCTCGAGATCAGTGTGTCATTGATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATCAATGACACACTGATCTCGAGATCAGTGTGTCATTGATCCCG-3'

Other clones with same target sequence:

(none)