Construct: shRNA TRCN0000120096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173731.1-261s1c1
DNA Barcode:
CCAAGGGATGGATTGCAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgcll1 (208982)
Gene Description:
3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1
Transcript:
RefSeq NM_173731.1 (NON-CURRENT)
Match location:
Position 261 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGGATGGATTGCAGAAT
Hairpin Sequence:
5'-CCGG-CCAAGGGATGGATTGCAGAAT-CTCGAG-ATTCTGCAATCCATCCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGGATGGATTGCAGAATCTCGAGATTCTGCAATCCATCCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGGATGGATTGCAGAATCTCGAGATTCTGCAATCCATCCCTTGG-3'

Other clones with same target sequence:

(none)