Construct: shRNA TRCN0000120103

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178413.3-575s1c1
DNA Barcode:
GCTGCCATTGAGAGTGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thnsl2 (232078)
Gene Description:
threonine synthase-like 2 (bacterial)
Transcript:
RefSeq NM_178413.3 (NON-CURRENT)
Match location:
Position 575 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120103)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCCATTGAGAGTGTTCAA
Hairpin Sequence:
5'-CCGG-GCTGCCATTGAGAGTGTTCAA-CTCGAG-TTGAACACTCTCAATGGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCCATTGAGAGTGTTCAACTCGAGTTGAACACTCTCAATGGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCCATTGAGAGTGTTCAACTCGAGTTGAACACTCTCAATGGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000163103