Construct: shRNA TRCN0000120185

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053235.1-524s1c1
DNA Barcode:
GAATGGTTGTAACAGTGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r13 (113862)
Gene Description:
vomeronasal 1 receptor 13
Transcript:
RefSeq NM_053235.1 (NON-CURRENT)
Match location:
Position 524 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120185)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGGTTGTAACAGTGACAA
Hairpin Sequence:
5'-CCGG-GAATGGTTGTAACAGTGACAA-CTCGAG-TTGTCACTGTTACAACCATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGGTTGTAACAGTGACAACTCGAGTTGTCACTGTTACAACCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGGTTGTAACAGTGACAACTCGAGTTGTCACTGTTACAACCATTC-3'

Other clones with same target sequence:

(none)