Construct: shRNA TRCN0000120297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019570.3-3999s1c1
DNA Barcode:
CAGCAGTGCTTGTGAGGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rev1 (56210)
Gene Description:
REV1, DNA directed polymerase
Transcript:
RefSeq NM_019570.3 (CURRENT)
Match location:
Position 3999 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAGTGCTTGTGAGGTATT
Hairpin Sequence:
5'-CCGG-CAGCAGTGCTTGTGAGGTATT-CTCGAG-AATACCTCACAAGCACTGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAGTGCTTGTGAGGTATTCTCGAGAATACCTCACAAGCACTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAGTGCTTGTGAGGTATTCTCGAGAATACCTCACAAGCACTGCTG-3'

Other clones with same target sequence:

(none)