Construct: shRNA TRCN0000120356

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011272.1-220s1c1
DNA Barcode:
CCATCCTTCATCAACAAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rln1 (19773)
Gene Description:
relaxin 1
Transcript:
RefSeq NM_011272.1 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120356)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCTTCATCAACAAAGAT
Hairpin Sequence:
5'-CCGG-CCATCCTTCATCAACAAAGAT-CTCGAG-ATCTTTGTTGATGAAGGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCTTCATCAACAAAGATCTCGAGATCTTTGTTGATGAAGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCTTCATCAACAAAGATCTCGAGATCTTTGTTGATGAAGGATGG-3'

Other clones with same target sequence:

  1. TRCN0000146706
  2. TRCN0000150355