Construct: shRNA TRCN0000120359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021545.1-285s1c1
DNA Barcode:
TGAATCTAGTTCAGCTGGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Naip7 (53880)
Gene Description:
NLR family, apoptosis inhibitory protein 7
Transcript:
RefSeq NM_021545.1 (CURRENT)
Match location:
Position 285 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAATCTAGTTCAGCTGGCAA
Hairpin Sequence:
5'-CCGG-TGAATCTAGTTCAGCTGGCAA-CTCGAG-TTGCCAGCTGAACTAGATTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAATCTAGTTCAGCTGGCAACTCGAGTTGCCAGCTGAACTAGATTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAATCTAGTTCAGCTGGCAACTCGAGTTGCCAGCTGAACTAGATTCA-3'

Other clones with same target sequence:

(none)