Construct: shRNA TRCN0000120442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009838.1-1657s1c1
DNA Barcode:
CCTGTGATACTACAGGATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cct6a (12466)
Gene Description:
chaperonin containing Tcp1, subunit 6a (zeta)
Transcript:
RefSeq NM_009838.1 (NON-CURRENT)
Match location:
Position 1657 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGATACTACAGGATGTT
Hairpin Sequence:
5'-CCGG-CCTGTGATACTACAGGATGTT-CTCGAG-AACATCCTGTAGTATCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGATACTACAGGATGTTCTCGAGAACATCCTGTAGTATCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGATACTACAGGATGTTCTCGAGAACATCCTGTAGTATCACAGG-3'

Other clones with same target sequence:

(none)