Construct: shRNA TRCN0000120476

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009840.2-962s1c1
DNA Barcode:
GCGGACATAGCTCTTCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cct8 (12469)
Gene Description:
chaperonin containing Tcp1, subunit 8 (theta)
Transcript:
RefSeq NM_009840.2 (NON-CURRENT)
Match location:
Position 962 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120476)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGACATAGCTCTTCATTAT
Hairpin Sequence:
5'-CCGG-GCGGACATAGCTCTTCATTAT-CTCGAG-ATAATGAAGAGCTATGTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGACATAGCTCTTCATTATCTCGAGATAATGAAGAGCTATGTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGACATAGCTCTTCATTATCTCGAGATAATGAAGAGCTATGTCCGC-3'

Other clones with same target sequence:

  1. TRCN0000320157