Construct: shRNA TRCN0000120486

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028283.1-2433s1c1
DNA Barcode:
CGGAGAACGATAAGTTGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uaca (72565)
Gene Description:
uveal autoantigen with coiled-coil domains and ankyrin repeats
Transcript:
RefSeq NM_028283.1 (NON-CURRENT)
Match location:
Position 2433 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120486)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAACGATAAGTTGACTA
Hairpin Sequence:
5'-CCGG-CGGAGAACGATAAGTTGACTA-CTCGAG-TAGTCAACTTATCGTTCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAACGATAAGTTGACTACTCGAGTAGTCAACTTATCGTTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAACGATAAGTTGACTACTCGAGTAGTCAACTTATCGTTCTCCG-3'

Other clones with same target sequence:

(none)