Construct: shRNA TRCN0000120498

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178884.2-1768s1c1
DNA Barcode:
GCGGTGAATTTGAGTGCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Obsl1 (98733)
Gene Description:
obscurin-like 1
Transcript:
RefSeq NM_178884.2 (NON-CURRENT)
Match location:
Position 1768 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120498)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGTGAATTTGAGTGCAGAA
Hairpin Sequence:
5'-CCGG-GCGGTGAATTTGAGTGCAGAA-CTCGAG-TTCTGCACTCAAATTCACCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGTGAATTTGAGTGCAGAACTCGAGTTCTGCACTCAAATTCACCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGTGAATTTGAGTGCAGAACTCGAGTTCTGCACTCAAATTCACCGC-3'

Other clones with same target sequence:

(none)