Construct: shRNA TRCN0000120502

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198411.1-2529s1c1
DNA Barcode:
GCTATGGCTGAGTTGAGGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Inf2 (70435)
Gene Description:
inverted formin, FH2 and WH2 domain containing
Transcript:
RefSeq NM_198411.1 (NON-CURRENT)
Match location:
Position 2529 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120502)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGGCTGAGTTGAGGTTA
Hairpin Sequence:
5'-CCGG-GCTATGGCTGAGTTGAGGTTA-CTCGAG-TAACCTCAACTCAGCCATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGGCTGAGTTGAGGTTACTCGAGTAACCTCAACTCAGCCATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGGCTGAGTTGAGGTTACTCGAGTAACCTCAACTCAGCCATAGC-3'

Other clones with same target sequence:

(none)