Construct: shRNA TRCN0000120503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198411.1-1780s1c1
DNA Barcode:
CCTCGAGTTCTCTAGCAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Inf2 (70435)
Gene Description:
inverted formin, FH2 and WH2 domain containing
Transcript:
RefSeq NM_198411.1 (NON-CURRENT)
Match location:
Position 1780 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGAGTTCTCTAGCAATAA
Hairpin Sequence:
5'-CCGG-CCTCGAGTTCTCTAGCAATAA-CTCGAG-TTATTGCTAGAGAACTCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGAGTTCTCTAGCAATAACTCGAGTTATTGCTAGAGAACTCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGAGTTCTCTAGCAATAACTCGAGTTATTGCTAGAGAACTCGAGG-3'

Other clones with same target sequence:

(none)