Construct: shRNA TRCN0000120532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026102.1-3700s1c1
DNA Barcode:
GCATTCATATTGAGTGGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Daam1 (208846)
Gene Description:
dishevelled associated activator of morphogenesis 1
Transcript:
RefSeq NM_026102.1 (NON-CURRENT)
Match location:
Position 3700 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCATATTGAGTGGGATT
Hairpin Sequence:
5'-CCGG-GCATTCATATTGAGTGGGATT-CTCGAG-AATCCCACTCAATATGAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCATATTGAGTGGGATTCTCGAGAATCCCACTCAATATGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCATATTGAGTGGGATTCTCGAGAATCCCACTCAATATGAATGC-3'

Other clones with same target sequence:

(none)