Construct: shRNA TRCN0000120550

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127837.2-2033s1c1
DNA Barcode:
CCTTCAGTTAAAGGGAAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fndc3a (319448)
Gene Description:
fibronectin type III domain containing 3A
Transcript:
RefSeq XM_127837.2 (NON-CURRENT)
Match location:
Position 2033 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120550)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCAGTTAAAGGGAAGATA
Hairpin Sequence:
5'-CCGG-CCTTCAGTTAAAGGGAAGATA-CTCGAG-TATCTTCCCTTTAACTGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCAGTTAAAGGGAAGATACTCGAGTATCTTCCCTTTAACTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCAGTTAAAGGGAAGATACTCGAGTATCTTCCCTTTAACTGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000339998